Shared imaging equipment in the Department of Cell and Molecular
Physiology includes a multiphoton Leica TCS SP5 on a fully motorized
inverted stand. This is a fully equipped instrument with the following
features: Tandem scanner system with conventional scanner optimized
for resolution (8192x8192) and a resonant scanner optimized for speed
(25 sustained frames/sec at 512x512), 8, 12, or 16 bit A:D.
Acousto-optical beam splitter with 5 channel fluorescence detection (3
conventional, 2 FLIM), 2-channel avalanche photodiodes for FCS,
2-channel non-descanned detectors for multiphoton imaging, a
transmitted detector for DIC, and a cooled CCD camera for widefield
imaging. Prism-based spectral emission from 400-800 nm. Super Z galvo
stage for fast Z-stack acquisition. Laser lines at 405, 458, 476,
488, 496, 514, 543, 594, 633 nm, plus a 4 watt tunable IR ultrafast
laser (Coherent Chameleon). Leica EL6000 lightsource for widefield
imaging. 12 assorted water/dry/oil objectives. Stage inversion optics
for dipping applications with an objective-mounted fast piezo
Z-controller. Tokai Hit GSI stage-top incubator system with mixed
C02/air and temperature control. Full software options including
deconvolution, linear unmixing, FRET, colocalization. Fully featured
offline software for analysis is available on an additional dedicated
workstation in the department. In addition, the microscope is equipped
with Becker and Hickl instrumentation for fluorescence correlation
spectroscopy and fluorescence lifetime imaging microscopy. FCS
hardware: A dedicated FCS computer including counting card and software
for single-photon counting. 2 channel APD detection, 7 assorted
dichroic sets for 2-channel separation. FLIM hardware: A dedicated
FLIM computer with B&H SPC830 acquisition board with 32 MB memory
for time-correlated single photon counting, electrical time resolution 8
ps FWHM, 4 ps RMS. Excitation with pulsed 405 or tunable IR laser,
detection with internal FLIM PMTs. Becker and Hickl triggering and
routing. System is housed a dedicated imaging facility (440 sq. ft)
with a wet bench and tissue culture incubator. |