Instruments and Methods

"Paul Bunyan" total internal reflection fluorescence (TIRF) and widefield epifluorescence microscope. Detection is with an iXon EMCCD camera.

 

"Babe the Blue Ox" TIRF and widefield microscope. This microscope is set up for laser spot photobleaching for FRAP and FTR. Babe also has a stage-top incubator and a stimulator for pacing. Detection is with an iXon EMCCD camera or Photometrics K4.


Example images of a HEK cell expressing YFP-Focal Adhesion Kinase excited with widefield and TIRF:



Laser beam combiner and wavelength selection device.