& Methods


Paul Bunyan

"Paul Bunyan" is a Total Internal Reflection Fluorescence (TIRF) and Widefield Epi-fluorescence microscope.

Detection is with an iXon EMCCD camera.


Babe the Blue Ox

"Babe the Blue Ox" is a TIRF and Widefield microscope. This microscope is set up for laser spot photo bleaching for FRAP and FTR.

Babe also has a stage-top incubator and a stimulator for pacing.

Detection is with an iXon EMCCD camera or Photometrics K4.

Example images of a HEK cell expressing YFP-Focal Adhesion Kinase excited with widefield and TIRF:


Laser beam combiner and

Wavelength selection device