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Instruments
Paul Bunyan
"Paul Bunyan" is a Total Internal Reflection Fluorescence (TIRF) and Widefield Epi-fluorescence microscope.
Detection is with an iXon EMCCD camera.
Babe the Blue Ox
"Babe the Blue Ox" is a TIRF and Widefield microscope. This microscope is set up for laser spot photo bleaching for FRAP and FTR.
Babe also has a stage-top incubator and a stimulator for pacing.
Detection is with an iXon EMCCD camera or Photometrics K4.
Example images of a HEK cell expressing YFP-Focal Adhesion Kinase excited with widefield and TIRF:
Methods
Laser beam combiner and
Wavelength selection device
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